under construction
| Dr. Nitsara Karoonuthaisiri Ph.D. Chemical Engineering, Stanford University This email address is being protected from spambots. You need JavaScript enabled to view it. |
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| Dr. Wanilada Rungrassamee Ph.D. Microbiology, University of Massachusetts Amherst This email address is being protected from spambots. You need JavaScript enabled to view it. |
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| Dr. Rungnapa Leelatanawit Ph.D. Biotechnology, Chulalongkorn University This email address is being protected from spambots. You need JavaScript enabled to view it. |
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| Dr. Ratthaphol Charlermroj Ph.D. Biology, Queen’s University Belfast This email address is being protected from spambots. You need JavaScript enabled to view it. |
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| Dr. Jarinthorn Teerapornpuntakit Ph.D. Physiology, Mahidol university This email address is being protected from spambots. You need JavaScript enabled to view it. |
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| Miss Umaporn Uawisetwathana MS. Microbiology, Mahidol University This email address is being protected from spambots. You need JavaScript enabled to view it. |
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Miss. Sawarot Maibunkaew |
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Miss Manlika Makornwattana |
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PhD students
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Miss Thitiporn Pattarakankul |
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Miss Piyawadee Rugsanit |
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Research output
Publication & Patent
| 2017 | |
| 1. | Leelatanawit, R., Uawisetwathana, U., Klanchui, A., Khudet, J., Phomklad, S., Wongtriphop, S., Jiravanichpaisal, P. and Karoonuthaisiri, N. (2017). Transcriptomic analysis of male black tiger shrimp (Penaeus monodon) after polychaete feeding to enhance testicular maturation. Marine Biotechnology, 19(2), 125–135. |
| 2. | Charlermroj, R., Makornwattana, M., Himananto, O., Seepiban, C., Phuengwas, S., Warin, N., Gajanandana, O. and Karoonuthaisiri, N . (2017). An accurate, specific, sensitive, high-throughput method based on a microsphere immunoassay for multiplex detection of three viruses and bacterial fruit blotch bacterium in cucurbits. Journal of Virological Methods, 247, 6-17. |
| 3. | Leelatanawit, R., Saetung, T., Phuengwas, S., Karoonuthaisiri, N. and Devahastin, S . (2017). Selection of reference genes for quantitative real-time PCR in postharvest tomatoes (Lycopersicon esculentum) treated by continuous low-voltage direct current electricity to increase secondary metabolites. International Journal of Food Science and Technology, 52(9), 1942–1950 . |
| 4. | Teerapornpuntakit, J., Chanprapaph, P., Karoonuthaisiri, N. and Charoenphandhu, N. (2017). Site-specific onset of low bone density and correlation of bone turnover markers in exclusive breastfeeding mothers. Breastfeeding Medicine, 12(6), 331-337. |
| 5. | Visudtiphole, V., Petkon, S., Sittikankaew, K., Sakayaroj, J. and Klinbunga, S. (2017). Calcium signaling-related genes in Penaeus monodon respond to abiotic stress and pathogenic bacteria common in aquaculture. Aquaculture, 479, 626-637. |
| 6. | Leelayuwapan, H., Kangwanrangsan, N., Chawengkirttikul, R., Ponpuak, M., Charlermroj, R., Boonyarattanakalin, K., Ruchirawat, S. and Boonyarattanakalin, S. (2017). Synthesis and immunological studies of the lipomannan backbone glycans found on the surface of Mycobacterium tuberculosis. Journal of Organic Chemistry, 82 (14), 7190–7199. |
| 7. | Mongkol, P., Bunphimpapha, P., Rungrassamee, W., Arayamethakorn, S., Klinbunga, S., Menasveta, P. and Chaiyapechara, S. (2018). Bacterial community composition and distribution in different segments of the gastrointestinal tract of wild-caught adult Penaeus monodon. Aquaculture Research, 49(1), 378–392 . |
| 8. | Uengwetwanit, T., Ponza, P., Sangsrakru, D., Wichadakul, D., Ingsriswang, S., Leelatanawit, R., Klinbunga, S., Tangphatsornruang , S. and Karoonuthaisiri, N. (inpress). Transcriptome-based discovery of pathways and genes related to reproduction of the black tiger shrimp (Penaeus monodon). Marine Genomics, doi.org/10.1016/j.margen.2017.08.007, . |
| 9. | Arayamethakorn, S., Karoonuthaisiri, N. and Rungrassamee, W. (2017). A multiplex bead-based assay for immune gene expression analysis in shrimp. Journal of Biotechnology, 260, 74-78. |
| 2015 | |
| 1. | Karoonuthaisiri, N., Charlermroj, R., Teerapornpuntakit, J., Kumpoosiri, M., Himananto, O., Grant, I.R., Gajanandana, O. and Elliott, C.T. (2015). Bead array for Listeria monocytogenes detection using specific monoclonal antibodies. Food Control, 47, 462–471. |
| 2. | Uawisetwathana, U., Graham, S.F., Kamolsukyunyong, W., Sukhaket, W., Klanchui, A., Toojinda, T., Vanavichit, A., Karoonuthaisiri, N. and Elliott, C.T. (2015). Quantitative 1H NMR metabolome profiling of Thai Jasmine rice (Oryza sativa) reveals primary metabolic response during brown planthopper infestation. Metabolomics, 11(6), 1640-1655. |
| 2014 | |
| 1. | Charlermroj, R., Himananto, O., Seepiban, C., Kumpoosiri, M., Warin, N., Gajanandana, O., Elliott, C.T. and Karoonuthaisiri, N. (2014). Antibody array in a multiwell plate format for the sensitive and multiplexed detection of important plant pathogens. Analytical Chemistry, 86 (14), 7049–7056. |
| 2. | Rungrassamee, W., Klanchui, A., Maibunkaew, S., Chaiyapechara, S., Jiravanichpaisal, P. and Karoonuthaisiri, N. (2014). Characterization of intestinal bacteria in wild and domesticated adult Black Tiger Shrimp (Penaeus monodon). PLOS one, 9(3), e91853. |
| 3. | Chirakul, S., Bartpho, T., Wongsurawat, T., Taweechaisupapong, S., Karoonutaisiri, N., Talaat, A.M., Wongratanacheewin, S., Ernst, R.K., Sermswan, R.W. (2014). Characterization of BPSS1521 (bprD), a regulator of Burkholderia pseudomallei virulence gene expression in the mouse model. PLOS one, 9(8), e104313. |
| 4. | Leelatanawit, R., Uawisetwathana, U., Khudet, J., Klanchui, A., Phomklad, S., Wongtripop, S., Angthoung, P., Jiravanichpaisal, P. and Karoonuthaisiri, N. (2014). Effects of polychaetes (Perinereis nuntia) on sperm performance of the domesticated black tiger shrimp (Penaeus monodon). Aquaculture, 433, 266–275. |
| 5. | Teerapornpuntakit, J., Klanchui, A., Karoonuthaisiri, N., Wongdee, K. and Charoenphandhu, N. (2014). Expression of transcripts related to intestinal ion and nutrient absorption in pregnant and lactating rats as determined by custom-designed cDNA microarray. Molecular and Cellular Biochemistry, 391(1-2), 103-116. |
| 6. | Thaitrong, N., Charlermroj, R., Himananto, O., Seepiban, C. and Karoonuthaisiri, N. (2013). Implementation of microfluidic sandwich ELISA for superior detection of plant pathogens . PLOS one, 8(12), e83231. |
| 7. | Wang, J., Morton, M.J., Elliott, C.T., Karoonuthaisiri, N., Segatori, L. and Biswal, S.L. (2014). Rapid detection of pathogenic bacteria and screening of phage-derived peptides using microcantilevers. Analytical Chemistry, 86(3), 1671-1678. |
| 8. |
Rungrassamee, W., Klanchui, A., Maibunkaew, S., Chaiyapechara, S., Jiravanichpaisal, P., Karoonuthaisiri, N. 2014. Characterization of intestinal bacteria in wild and domesticated adult black tiger shrimp (Penaeus monodon). PlosOne (accepted)
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| 9. |
Karoonuthaisiri, N. Charlermroj, R., Morton, M.J., Oplatowska, M., Grant, I.R., Elliott, C.T. 2014Development of a M13 Bacteriophage-based SPR detection assay using Salmonella detection as a case study. Sens. Actuators B: Chem 190: 214– 220
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| 10. | Rungrassamee, W., Kingcha, Y., Srimarut, Y., Maibunkaew, S., Karoonuthaisiri, N. and Visessanguan, W. (2014). Mannooligosaccharides from copra meal improves survival of the Pacific white shrimp (Litopenaeus vannamei) after exposure to Vibrio harveyi. Aquaculture, 434, 403–410. |
| 2013 | |
| 1. |
Thaitrong, N., Charlermroj, R., Himanato, O., Seepiban, C., Karoonuthaisiri, N. 2013. Implementation of Microfluidic Sandwich ELISA for Superior Detection of Plant Pathogens. Plos ONE 8(12): e83231.
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| 2. |
Morton, M.J., Karoonuthaisiri, N., Charlermroj, R., Stewart, L.D., Elliott, C.T., Grant, I.R. 2013. Phage display-derived binders able to distinguish Listeria monocytogenes from other Listeria species. Plos One 8(9): e74312.
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| 3. |
Rungrassamee. W., Maibunkaew, S., Karoonuthaisiri, N.,Jiravanichpaisal, P. 2013. Application of bacterial lipopolysaccharide to improve survival of the black tiger shrimp after Vibrio harveyi exposure. Developmental and Comparative Immunology 41(2): 257 - 262
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| 4. |
Morton, M.J., Karoonuthaisiri, N., Stewart, L.D., Oplatowska, M., Elliott, C.T., Grant, I.R. 2013. Production and evaluation of the utility of novel phage display-derived peptide ligands to Salmonella spp. for magnetic separation. J Appl Microbiol 115: 271--281
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| 5. |
Charlermroj, R., Oplatowska, M., Gajanandana, O., Himananto, O., Grant, I.R., Karoonuthaisiri, N., Elliott, C.T. 2013. Strategies to Improve the Surface Plasmon Resonance-Based Immmunodetection of Bacterial Cells. Microchimica Acta 180(7): 643-650
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| 6. |
Charlermroj, R., Himananto, O., Seepiban, C., Kumpoosiri, M., Warin, N., Oplatowska, M., Gajanandana, O., Grant, I.R., Karoonuthaisiri, N., Elliott, C.T. 2013. Multiplex Detection of Plant Pathogens Using a Microsphere Immunoassay Technology. PloS ONE 8(4):e62344.
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| 7. |
Rungrassamee. W., Klanchui. A., Chaiyapechara, S., Maibunkaew, S., Tangphatsornruang, S., Jiravanichpaisal, P., Karoonuthaisiri, N. 2013. Bacterial population in intestines of the black tiger shrimp (Penaeus monodon) under different growth stages. PLos ONE 8(4):e60802.
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| 2012 | |
| 1 |
Leelatanawit, R., Klanchui, A., Uawisetwathana, U., Karoonuthaisiri, N. 2012. Validation of reference genes for real-time PCR of reproductive system in the black tiger shrimp. PLos ONE 7(12): e52677.
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| 2. |
Rungrassamee, W., Tosukhowong, A., Klanchui, A., Plengvidhya, V., Karoonuthaisiri, N. 2012. Development of bacteria identification array to detect the lactic acid bacteria in Thai fermented sausage J. Micro Methods 91:341–353.
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| 3. |
Ruktanonchai, U., Nuchuchua, O, Charlermroj, R., Pattarakankul, T, Karoonuthaisiri, N. 2012. Signal Amplification of Microarray-based Immunoassay by Optimization of Nanoliposome Formulations. Analytical Biochemistry 429(2):142-7.
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| 4. |
Bartpho, T., Wongsurawat, T., Wongratanacheewin, S., Talaat, A.M., Karoonuthaisiri, N., Sermswan, R.W. 2012. Genomic islands as a marker to differentiate between clinical and environmental Burkholderia pseudomallei. PLoS ONE 7(6): e37762.
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| 5. |
Oaew, S, Charlermroj, R, Pattarakankul, T, Karoonuthaisiri, N 2012. Gold nanoparticles/horseradish peroxidase encapsulated polyelectrolyte nanocapsule for signal amplification in Listeria monocytogenes detection. Biosens Bioelectron. 15;34(1):238-43.
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| 6. |
Charlermroj, R., Oplatowska, M., Kumpoosiri, M., Himananto, O., Gajanandana, O., Elliott, C., Karoonuthaisiri, N. 2012. Comparison of techniques to screen and characterize bacteria-specific hybridomas for high quality monoclonal antibodies selection. Analytical Biochemistry 421(1):26-36.
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| 7. |
Chaiyapechara, S., Rungrassamee, W., Suriyachay, I., Kuncharin, Y., Klanchui, A., Karoonuthaisiri, N., Jiravanichpaisal, P. 2012. Bacterial community associated with the intestinal tract of P. monodon in commercial farms. Microbial. Ecology. 63(4):938-53
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| 2011 | |
| 1. |
Uawisetwathana, U., Leelatanawit, R., Klanchui, A., Prommoon, J., Klinbunga, S., Karoonuthaisiri, N 2011 Insights into Eyestalk Ablation Mechanism to Induce Ovarian Maturation in the Black Tiger Shrimp. PLoS ONE 6(9): e24427
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| 2. |
Charlermroj, R., Gajanandana, O., Barnett, C., Kirtikara, K., Karoonuthaisiri, N. 2011. A chemiluminescent antibody array system for detection of foodborne pathogens in milk. Analytical Letters 44: 1–15.
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| 3. |
Wongsurawat, T., Leelatanawit, R., Thamniemdee, N., Uawisetwathana, U., Karoonuthaisiri, N., Menasveta, P., Klinbunga, S. 2010 Identification of testis-relevant genes using in silico analysis from testes ESTs and cDNA microarray in the black tiger shrimp (Penaeus monodon). BMC Molecular Biology, 11, 55.
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| 2010 | |
| 1. |
Rungrassamee, W., Leelatanawit, R., Jiravanichpaisal, P., Klinbunga, S., Karoonuthaisiri, N 2010. Expression and distribution of three heat shock protein genes under heat shock stress and under exposure to Vibrio harveyi in Penaeus monodon. Developmental and Comparative Immunology 34: 1082–1089.
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| 2. |
Brück, T., Beaudry, C., Hilgenkamp, H., Karoonuthaisiri, N, Salah-Eldin Mohamed, H., Weiss, G. A. 2010. Empowering Young Scientists. Science. 328: 17.
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| 3. |
Soontornchai, W., Rungrassamee, W., Karoonuthaisiri, N, Klinbunga, S., Söderhäll, K., Jiravanichpaisal, P. 2010. Expression of immune-related genes in the digestive organ of shrimp, Penaeus monodon, after an oral infection by Vibrio harveyi. Developmental and Comparative Immunology 34:19-28.
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| 2009 | |
| 1. |
Oaew, S., Karoonuthaisiri, N, Surareungchai, W. 2009. Sensitivity Enhancement in DNA hybridization Assay using gold nanoparticle-labeled two reporting probes. Biosensors Bioelectronics.25: 435–441.
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| 2. |
Karoonuthaisiri, N., Charlermroj, R., Uawisetwathana, U., Luxananil, P., Kirtikara, K., Gajanandana, O. 2009. Development of Antibody Array for Simultaneous Detection of Foodborne Pathogens. Biosensors Bioelectronics24:1641-1648.
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| 3. |
Karoonuthaisiri, N., Sittikankaew, K., Preechaphol, R., Wongsurawat, T., Uawisetwatthana, U., Kalachikov, S., Russo, J.J., Ju, J., Klinbunga, S., Kirtikara, K. 2009. ReproArrayGTS: A cDNA microarray for identification of reproduction-related genes in the giant tiger shrimp Penaeus monodon and characterization of a novel nuclear autoantigenic sperm protein (NASP) gene. Comp Biochem Physiol Part D 4(2): 90-99.
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| 2008 | |
| 1. |
Charlermroj, R., Uawisetwathana, U., Luxananil, P., Kirtikara, K., Gajanandana, O., Karoonuthaisiri, N. 2008. Development of foodborne pathogen antibody array. Journal of Biotechnology 136S: S753
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| 2. |
Uawisetwathana, U., Sittikankeaw, K., Preechapol, R., Wongsurawat, T., Kalachikov, S., Russo, J.J., Ju, J., Klinbunga, S., Kirtikara, K., Karoonuthaisiri, N. 2008. Construction of reproduction-specific cDNA microarrays for the black tiger shrimp (Penaeus monodon). Journal of Biotechnology 136S: S546
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| 3. |
Charlermroj, R., Uawisetwathana, U., Luxananil, P., Kirtikara, K., Gajanandana, O., Karoonuthaisiri, N. 2008. Development of foodborne pathogen antibody array. Journal of Biotechnology 136S: S753
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| 4. |
Pacharawongsakda, E., Yokwai1, S., Karoonuthaisiri, N., Wichadakul, D., Ingsriswang, S. 2008. ESTplus: An Integrative System for Comprehensive and Customized EST Analysis and Proteomic Data Matching. IEEE. 29-32.
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| 2007 | |
| 1. |
Shaw, P.J., Ponmee, N., Karoonuthaisiri, N.,Kamchonwonpaisan, S., Yuthavong, Y. 2007. Characterization of human malaria parasite Plasmodium falciparum eIF4E homologue and mRNA 5’cap status. Molecular and Biochemical Parasitology, 155: 146-155.
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| 2005 | |
| 1. |
Karoonuthaisiri, N, Weaver, D., Huang J., Cohen S.N., Kao C.M. 2005. Regional organization of gene expression in Streptomyces coelicolor. Gene. 353: 53-66.
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| 2. |
Lee, E., Karoonuthaisiri, N, Kim, H., Park, J., Kao, C.M., Roe, J. 2005. A master regulator sB that governs osmotic and oxidative response as well as differentiation via sigma cascade in Streptomyces coelicolor. Molecular Microbiology. 57(5): 1252-1264.
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| 3. |
Huang, J., Shi, J., Molle, V., Sohlberg, B., Weaver, D., Bibb, M.J., Karoonuthaisiri, N, Lih, C., Kao, C.M., Buttner, M.J., Cohen, S.N. 2005. Cross-regulation among disparate antibiotic biosynthetic pathways of Streptomyces coelicolor. Molecular Microbiology. 58(5): 1276-1287.
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| 2004 | |
| 1. |
Weaver, D., Karoonuthaisiri, N., Tsai H., Huang C., Ho M., Gai S., Patel K.G., Huang J., Cohen S.N., Hopwood D.A., Chen C., Kao C.M. 2004. Genome plasticity in Streptomyces: identification of 1 MbTIRs in the S. coelicolor A3(2) chromosome.. Molecular Microbiology. 51(6): 1535-1550.
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| 2. |
Karoonuthaisiri, N, Titievskiv, K., Thomas, J.L. 2003. Destabilization of Fatty Acid-Containing Liposomes by Polyamidoamine Dendrimers. Colliods and Surfaces B: Biointerface. 27: 365-75.
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| 3. |
Elliot, M.A., Karoonuthaisiri, N, Huang, J., Cohen, S., Bibb, M.J., Kao, C.M., Buttner, M.J. 2003. The Chaplins: A Family of Hydrophobic Cell-surface Proteins Involved in Aerial Mycelium Formation in Streptomyces coelicolor. Genes & Development. 17:1727-40.
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PATENT
- Karoonuthaisiri, N, Charlermroj, R., Uawisetwathana, U., Luxananil, P., Kirtikara, K., Gajanandana,
- O. Method for foodborne pathogen detection using antibody array
- (filed in Thailand in July 14, 2008: No 0801003632)
- Karoonuthaisiri, N, Charlermroj, R., Kirtikara, K., Gajanandana, O.
- Antibody array chip for multiplex detection of foodborne pathogens
(filed in Thailand in June 4, 2009: No 0901002489) - Oaew, S., Karoonuthaisiri, N., Surareungchai, W.
- Method for DNA detection using two gold-nanoparticle probes
(filed in Thailand in July 2, 2009: No 0901002999) - Karoonuthaisiri, N, Charlermroj, R., Himanunto, O., Kumpoosiri, M., Gajanandana, O.
Methode for hybridoma screening by antibody array using whole-cell bacteria labeled with fluorescent dye in solution as a reporter (filed in Thailand in September 24, 2009: No 0901004302) - Karoonuthaisiri, N, Charlermroj, R., Himanunto, O., Kumpoosiri, M., Gajanandana, O.
Method for hybridoma screening by antibody array using whole-cell bacteria coated on a solid surface and a fluorescently labeled anti-mouse antibody as a reporter (filed in Thailand in September 24, 2009: No 0901004303) - Rungrassamee, W., Karoonuthaisiri, N, Wongsurawat, T., Plengvidhya, V.
Method for identification of lactic acid bacteria by DNA microarray technique
(filed in Thailand in July 28, 2010: No 1001001146) - Oaew, S., Karoonuthaisiri, N., Charlermroj, R., Pattarakankul, T.
Preparation method and applications of antibody labeled with an enzyme encapsulation
(filed in Thailand in August 18, 2011: No 1101001600)
PETITE PATENT
- Karoonuthaisiri, N., Ruktanonchai,U., Nuchuchua, O., Charlermroj, R., Pattarakankul, T.
Method for Listeria monocytogenes detection based on antibody array using liposome encapsulation conjugated with antibodies and enzyme for naked eye signal detection (filed in Thailand in August 18, 2011: No 1103000847) - Rungrassamee, W., Maibunkaew, S., Karoonuthaisiri, N. Primers for specific identification of Vibrio
harveyi (filed in Thailand in September 13, 2012: No 1203000962
| Research & Development | ||
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Three main research and development areas are our main focus:
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| 1. | cDNA microarray technology | |
| a. | We have constructed and employed several versions of cDNA Microarrays to study the black tiger shrimp in different aspects namely reproductive maturation of broodstock, nutrigenomics, and stress responses. | |
| 2. | Development of diagnostics | |
| a. | Microarray-based diagnostics for food pathogens and plant pathogens | |
| b. | Bead-based microarray to detect food pathogens, plant pathogens, calcium biomarkers | |
| c. | DNA microarray to detect bacteria community in the shrimp intestine to understand the dynamics of these bacteria and how they affect the well-being of the shrimp. | |
| 3. | Exploration of other technology platforms with high-throughput capacities for research and development | |
| a. | Metabolomic analysis to unravel markers for aroma rice and brown planthopper resistant rice. | |
| Vision | ||
| To be a leading research group for employing microarray and emerging high-throughput technologies for fundamental and translational research and development. | ||
| Mission | ||
| 1 | Development of DNA microarray to study economically important black tiger shrimp in order to revive the shrimp industry of Thailand | |
| 2 | Development of microarray-based diagnostics for agriculture and food applications. | |
| 3 | Development of new high-throughput technologies such as metabolomic techniques and bead array for fundamental and applied research. | |